Autoradiograms of steroid metabolites out-of vitellogenic ovarian follicles of your own bambooleaf wrasse incubated with [7- step 3 H]pregnenolone

Autoradiograms of steroid metabolites out-of vitellogenic ovarian follicles of your own bambooleaf wrasse incubated with [7- step 3 H]pregnenolone

Eight metabolites was broke up by narrow level chromatography and set up having benzene:acetone (4:1). step 1, E1; 2, AD; step 3, P5; 4, DHEA 17-P; 5, E2; six, 1seven-P5; eight, 17,20?-P 17,21-P; and you can 8, 17,20?-P5. See Table step 1 to possess steroid abbreviations.

Desk step three

To search for the steroidogenic path out-of E2 synthesis, five most other radiolabeled steroid drugs were used since precursors having for the vitro incubation off hair follicles. Of them four precursors, E2 are synthesized out of [ fourteen C]DHEA, [ 14 C]Advertisement, and [ 3 H]E1. Whenever follicles was indeed incubated having [ step 3 H]17-P, E2 wasn’t sensed since the metabolite, and you can a small amount of Advertisement was delivered. Throughout incubations, T production could not getting imagined, and you can [ fourteen C]T was not entirely changed into E2.

Dimension out-of gel steroid levels

Serum amounts of E2 and you may E1 showed comparable users from inside the a beneficial day’s spawning year (Fig. dos). On hours, if the developmental phase of the prominent oocytes is actually LMN, gel E2 and you may E1 levels had been notably highest (E2= ng/ml, E1= 5.08 ng/ml). However, because of the hr, in the event that phase of one’s premier oocytes was Meters, the amount dropped (E2=5.03 ng/ml, E1=step one.fifty ng/ml) and you can remained ongoing account on and you will hours, in the event that grade of the prominent oocytes was in fact TY and you can EMN, correspondingly (E2=six.00 ?six.twenty seven ng/ml, Estep one=1.15–step one.79 ng/ml). not, T and you will Offer profile was lingering (T=step 1.22–1.71 ng/ml, AD=step 1.23–step one.43 ng/ml) throughout the day and failed to disagree significantly certainly ovarian level (Fig. step 3).

Fig. 2

Changes in the serum levels of estradiol-17? (E2) and estrone (E1) in female bambooleaf wrasse sampled at different times of a day during the spawning season. Numbers indicate sample size. Each value is the mean±SEM. Statistically significant differences between groups are shown by different letters (P<0.05).

Fig. 3

Changes in gel degrees of testosterone (T) and you may androstenedione (AD) in females bambooleaf wrasse tested in the different times out of a day inside the spawning 12 months. Quantity imply sample size. For every single well worth is the imply±SEM.


This research reveals the fresh steroidogenic pathway to help you E2 synthesis about ovarian hair follicles away from bambooleaf wrasse (Fig. 4, vitellogenic path). In the last 20 years, a good amount of studies to the steroidogenesis throughout the vitellogenesis, in numerous teleosts, enjoys incubated unchanged ovarian follicles or fragments otherwise measured bloodstream steroid account. when you look at the vitro incubation away from ovarian follicles (amago salmon Oncorhynchus rhodurus , Kagawa et al., 1982; goldfish, Kagawa et al., 1984; guppy Poecilia reticulata , Venkatesh et al., 1992; medaka Oryzias latipes, Kobayashi mais aussi al., 1996) or ovarian fragments (elizabeth.grams., Atlantic croaker Micropogonias undulatus , Trant and you will Thomas, 1989) exhibited that E2 are synthesized through T. In almost any varieties, this has been shown you to bloodstream E2 membership increase during vitellogenesis, as well as T accounts (age.g., plaice Pleuronectes platessa , Wingfield and you can Grimm, 1977; light sucker Catostomus commersori , Scott et al., 1984; noticed seatrout Cynoscion nebulosus , Thomas et al., 1987; red-colored seabream Pagrus significant, Matsuyama mais aussi al., 1988; gulf of mexico killifish Fundulus grandis , Greeley mais aussi al., 1988; sea trout Dicentrarchus labrax , Prat mais aussi al., 1990; demoiselles Chromis dispilus , Barnett and you will Pankhurst, 1994; striped bass Morone saxatilis , Queen ainsi que al., 1994; spiny damselfish Acanthochromis polyacanthus , Pankhurst et al., 1999). These studies provides added me to this new approved evaluate that T is a precursor having E2 design in the teleost ovary. Inside our analysis, but not, T was not produced by vitellogenic follicles immediately following incubation which have radiolabeled precursors, and you will exogenous T wasn’t converted to E2 regarding hair follicles. These show clearly imply that inside bambooleaf wrasse T is not brand new forerunner to own E2 production throughout the vitellogenic hair follicles. Conversely, E1 are pure ücretsiz produced rather than T away from P5, DHEA, and Advertising, and you can exogenous E1 try translated into E2. The outcomes suggest that E2 are synthesized out of E1 on the vitellogenic hair follicles. Moreover, the new serum steroid membership, where gel E2 levels exhibited an effective diurnal rhythm one paralleled E1 accounts although not T accounts, keep the inside the vitro research. To your degree, this is actually the first claim that E1 ‘s the significant sandwich-stratum from E2 on the ovarian follicles when you look at the an effective teleost.

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